Phosphate Buffer, pH 7.4 [0.5 M] - 250 ml
Our comprehensive range of biological buffers is essential for research, diagnostics, drug development, and manufacturing.
Phosphate Buffer, pH 7.4 [0.5 M] - 250 ml
منتج #: 210032152

Phosphate Buffer, pH 7.4 [0.5 M] - 250 ml

منتج #: 210032152

JOD 45

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Our comprehensive range of biological buffers is essential for research, diagnostics, drug development, and manufacturing.
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مايفيد

Optimal pH Stability
This phosphate buffer maintains a consistent pH of 7.4, ideal for various biological and chemical applications, ensuring reliable and reproducible results in laboratory experiments.
High Concentration
With a concentration of 0.5 M, this buffer offers effective stabilization for sensitive reactions, enhancing performance in protocols compared to lower concentration alternatives.
Convenient Volume
Available in a 250 ml size, it provides ample quantity for multiple experiments, minimizing waste and ensuring long-lasting usage, perfect for research labs and educational settings.

تفاصيل المنتج

Shop Phosphate Buffer, pH 7.4 [0.5 M] - 250 ml online at a best price in الاردن. B09V5KBTQP
Container TypeBottle
Item FormLiquid
Item Weight8 ounces (226.8 grams)
Volume250 ml
Brand NameeCon Lab Supply
ManufacturerCiro Mfg
Included ComponentsBottle
Part Number23.105.81
BrandeCon Lab Supply
Item Volume250 ml
Package InformationBottle

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Suitable For
  • Biological Research

    Essential for cell culture and biochemical assays, maintaining stable pH for optimal enzyme activity and molecular stability.

  • Pharmaceutical Applications

    Ideal for drug formulation studies, ensuring consistent pH during the development and testing phases of pharmaceutical products.

  • Laboratory Use

    A must-have reagent for routine experiments involving protein purification and DNA/RNA analysis, providing precise pH control.

Not Suitable For
  • Food Industry

    Not suitable for food preservation or processing, as the dosage and handling do not meet food safety standards.

وصف المنتج

Phosphate Buffer, pH 7.4 [0.5 M] - 250 ml

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أسئلة العملاء & الإجابات

  • سؤال: What is Phosphate Buffer, PH 7.4 used for?

    إجابه: Phosphate Buffer, PH 7.4 is primarily used in biological and chemical applications, particularly in molecular biology and biochemistry labs. It helps maintain a stable pH environment, which is crucial for enzyme reactions, cell cultures, and various biochemical assays. By providing this stable pH, researchers can achieve more consistent and reproducible results in experiments where pH fluctuations could affect outcomes.
  • سؤال: Why is a pH of 7.4 important in buffers?

    إجابه: A pH of 7.4 is essential because it closely resembles the physiological pH of human blood. Many biological processes and enzyme activities depend on this specific pH for optimal functioning. Using a buffer with this pH helps to simulate body conditions in lab experiments, making it ideal for studying biological reactions or testing pharmaceuticals. This relevance makes it a go-to solution for researchers and laboratories working in life sciences.
  • سؤال: Can I use Phosphate Buffer for protein purification?

    إجابه: Yes, Phosphate Buffer, PH 7.4 is often used in protein purification protocols. It provides the necessary ionic composition and pH stability that help maintain protein solubility and functionality during purification processes like chromatography or precipitation. Additionally, because it minimizes protein denaturation, it is particularly beneficial when working with sensitive biomolecules, ensuring higher yield and purity of the final product.
  • سؤال: Is Phosphate Buffer effective for cell culture applications?

    إجابه: Absolutely! Phosphate Buffer, PH 7.4 is frequently employed in cell culture applications due to its ability to maintain a consistent pH level that is vital for cell viability and growth. Maintaining physiological pH helps support cellular processes, thus leading to more reliable experimental results. It’s commonly used in media formulations to stabilize the environment that cells operate in during experiments.
  • سؤال: How should I store the Phosphate Buffer once opened?

    إجابه: After opening, Phosphate Buffer, PH 7.4 should be stored in a cool, dark place, preferably at room temperature or chilled according to storage guidelines. It should be tightly sealed to prevent contamination or evaporation. For long-term use, refrigeration may be advisable, but always check the specific manufacturer’s guidelines to ensure the buffer's effectiveness remains intact for future experiments.
  • سؤال: What are the typical applications of this buffer in research?

    إجابه: Phosphate Buffer, PH 7.4 is widely utilized in various research applications, including enzyme kinetics, immunoassays, DNA/RNA isolation, and protein folding studies. Researchers often use it to maintain pH levels in reactions involving nucleic acids, enzymes, and antibodies. Its versatility makes it an essential component in many laboratory settings focused on biochemical and molecular biology studies.
  • سؤال: How do I prepare a phosphate buffer solution?

    إجابه: To prepare a Phosphate Buffer, PH 7.4 solution, start with a stock solution of monobasic and dibasic sodium phosphate. Mix the two in appropriate proportions to adjust the pH to 7.4 using a pH meter. It's crucial to ensure all reagents are of analytical grade for consistency and reliability in experiments. Following preparation, it's advisable to test the pH again before use to confirm its accuracy for research purposes.
  • سؤال: Is Phosphate Buffer safe to handle?

    إجابه: Phosphate Buffer is generally considered safe for handling in laboratory settings. However, standard safety precautions should always be taken, such as wearing gloves and protective eyewear, especially when working with concentrated solutions or fresh preparations. While it poses low toxicity, it's important to be cautious to avoid accidental ingestion or contact with eyes or skin.
  • سؤال: Can this buffer be used in clinical diagnostics?

    إجابه: Yes, Phosphate Buffer, PH 7.4 is used in various clinical diagnostic tests, particularly where biological components are analyzed. Its stable pH can enhance the reliability of test results, making it suitable for immunological assays, enzyme tests, and more. Its ability to maintain a consistent environment is vital for providing accurate diagnoses in clinical laboratories focused on patient care.
  • سؤال: Where can I buy Phosphate Buffer, PH 7.4 [0.5 M] - 250 Ml?

    إجابه: You can purchase Phosphate Buffer, PH 7.4 [0.5 M] - 250 Ml from Ubuy. They offer a wide selection of biochemical reagents, including high-quality phosphate buffers, ensuring you have access to the products you need for your laboratory or research work in Jordan.

eCon Lab Supply Buffers Editorial Review

Phosphate Buffer, pH 7.4 [0.5 M] - 250 ml is a reliable liquid buffer solution packaged in a convenient bottle from eCon Lab Supply. With a composition that ensures stability at a pH of 7.4, this buffer solution is ideal for various laboratory applications where maintaining pH is crucial. The item weight is 8 ounces (226.8 grams), making it easy to handle and store. Users appreciate the simplicity of the packaging and the effectiveness of the solution in experiments. The inclusion of a well-sealed bottle ensures that the solution remains uncontaminated, contributing to consistent results in lab settings.

مراجعات العملاء وتقييماتهم

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إيجابيات

  • Convenient 250 ml liquid buffer for various applications
  • Stable pH of 7.4 ideal for experiments
  • Easy to use bottle container
  • Lightweight for handling and storage
  • Effective for maintaining pH in laboratory settings

سلبيات

  • Limited information on specific use cases and compatibility

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